Equipment is located at Wolf Hall Room 007, 245 and at the Helen F. Graham Cancer Center, Suite 4300.
FACSCalibur Flow Cytometer
The BD FACSCalibur flow cytometer is a four-color, dual-laser benchtop system capable of cell analysis as well as cell sorting. It supports a wide assortment of applications in including clinical and environmental research environments. To access the FACSCalibur machine, clients are asked to contact the CTCR Core Facility in advance to set up a training and consultation module or to demonstrate competency in use of the equipment. Use of the instrument is available based on signing up on the CTCR Core Facility web calender, on an as-available basis on a for-fee schedule. Hourly, yearly and long-term usage fees are available for clients to accommodate researchers and their needs. ***Tubes required for use are the BD Falcon* Tube with Cell Strainer Cap, Part No.:352235.
FACSAria II Flow Cytometer
The BD FACSAriaII flow cytometer is a six- color, dual-laser (488 and 640nm) benchtop system capable of cell analysis and can sort up to four populations. It supports a wide assortment of applications in including clinical and environmental research environments. Clients can access the FACSAriaII machine at the Helen F Graham Cancer Center, Suite 4300. Use of the instrument is available based on signing up on the CTCR Core Facility web calender, on an as- available and day available basis on a for-fee schedule.
Accuri C6 Flow Cytometer The BD Accuri™ C6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable. The system is equipped with a blue and a red laser, two light scatter detectors, and four fluorescence detectors with optical filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. A compact optical design, fixed alignment, and pre-optimized detector settings make the system easier to use. Clients can access the machine at Wolf Hall room 245. Use of the instrument is available based on signing up on the CTCR Core Facility web calender, on an as- available and day available basis on a for-fee schedule.
IVIS Lumina System
The Caliper IVIS Lumina provides high sensitivity, ease of use, and the flexibility to image fluorescent and bioluminescent reporters both in vivo and in vitro. The system includes a highly sensitive CCD camera (thermoelectrically (Peltier) cooled to -90 οC with 1024 x 1024 pixels 13 micron in width), a light-tight imaging chamber with complete computer automation (High light collection lens, f /0.95 – f/16), 8 position emission filter wheel, LED lamps for photographic images, and the Living Image software package for image acquisition and analysis.
Other features include an adjustable field of view from 5 – 12.5 cm allows imaging of up to 3 mice or a medium size rat and also accommodates standard microtiter plates for in vitro imaging. The system includes premium animal handling features such as a heated stage, gas anesthesia connections and 5 position manifold within imaging chamber allow anesthesia to be maintained during imaging, and an optional full gas anesthesia. The Caliper IVIS is also used for imaging fluorescent and bio- luminescent reporters in plants, soils and other non-animal samples.
ABI Prism 7000 and 7300 real time PCR Systems
The ABI Prism 7000 and 7300 Real Time PCR systems combines thermal cycling, fluorescence detection, and application-specific software to detect the accumulation of target product cycle-by-cycle during PCR. Using flourogenic TaqMan® probes or SYBR® Green 1 dye chemistries, the 7000 and 7300 systems can provide real-time quantitation of DNA or RNA by monitoring the exponential phase of the PCR reaction. Applications for real-time PCR technology include quantitative gene expression analysis, pathogen detection/ quantitation, allelic discrimination (SNP detection), and mutation screening.
ABI Primer Express® Software is the recommended method for primer/probe design. Reagents and consumables (including SYBR® Green PCR kits and 96-well plates) may be ordered directly from Applied Biosystems.
DTX-880 Plate Reader
The Beckman Coulter DTX 880 plate reader features fluor- essence intensity, absorbance and glow luminescence for 96- to 384-well plates, as well as linear and orbital shaking. Users can program endpoint, kinetic, multiple wavelength, linear scan and area scan measurement types. Easily accessible six-position filter slides enable wavelength selection from 340 nm to 650 nm.
Glomax Multi-Modal Plate Reader with Injectors
Glomax multimodal can run fluorescence, luminescence and absorbance on 6, 12, 24, 48, 96, and 384-well plate layouts. It can also heat from 2° above ambient temperature to 45° C and allows for linear or orbital shaking. Single or dual auto injectors are allow for flash-based luminescence applications or dual-reporter assays. Each injector system has a volume range of 25 - 200 µl in 5µl increments for 6- to 96-well plate formats.
The AlphaImager HP high performance imaging system delivers an easy to operate imaging technology with simple, intuitive software and real time imaging. When high resolution is desired for fluorescent and visible imaging applications, the AlphaImager HP imaging system is the documentation and quantitative analysis system of choice. The AlphaImager HP is housed in our flexible MultiImage II cabinet. This versatile, upgradeable cabinet houses a dual wavelength, pull out UV transilluminator, white light table, EPI white lights and optional dual wavelength Epi UV lights. The filter wheel allows for imaging of fluorescent dyes emitting both 537nm and 545nm.
Fluorochem Q Chemi-Imager
The multiplex fluorescent detection and chemiluminescent imaging capabilities of the FluorChem Q provide a complete solution for quantitative Western blot imaging and analysis. The open platform is compatible with a wide variety of dyes and kits, providing the flexibility needed for multiple applications. The FluorChem Q allows quantitative detection of multiple proteins in a single assay, eliminating the need to strip and re-probe blots. Intensity values are easily normalized to a loading control to correct for inaccuracies. The Fluorocehm system has a six-position filter wheelwhich provides UV and fluorescent imaging versatility; Three excitation channels with specific wavelengths for multiplex fluorescent Western blot imaging; A powerful fixed lens for rapid imaging speeds; AlphaView® Q software which saves imaging protocols and provides quantitative analysis tools.
A microtome can be used to create extremely thin sections of a sample, in preparation for observations under light or electron microscopes. Microtomes use steel, glass or diamond blades depending in the sample being prepared and the desired section thickness.
Leica RM2125 Microtome
The Leica RM2125 microtome can be used to quickly section samples.
Tissue Culture Hood
The tissue culture hood is a sterile environment for sample preparation and experiment processing.
3D Amira Workstation, Mac/PC:
Amira 5, Volocity 4, AIM, Photoshop CS4, Illustrator, and ImageJ-64bit.
Amira is a powerful, multi- faceted software platform for visualizing, manipulating, and understanding Life Science and bio-medical data coming from all types of sources and modalities. Software features include ortho and oblique slicing, volume and surface rendering, isolines, molecular visualization, skeletonization, deconvolution, animation, movie generation, 3D stereo vision and many others. Computer workstation is a Windows capable Mac Pro, 640GB HD with an Apple Cinema HD Display (30" flat panel) with 2560 x 1600 pixel resolution for optimal working conditions. Windows software: LSM Image Examiner, Zen Light Edition, VLC Media Player, ImageJ, myVGL 2.0, ABI Primer Express 3.0, Microscoft Office (Excel, Word, PowerPoint, Publisher), Adobe Acrobat, Firefox, Internet Explorer and iTunes. Macintosh software: Amira, ImageJ, Adobe Photoshop, Adobe Illustrator, Adobe Acrobat Pro, Omni Outliner, Keynote, Pages, Numbers, Microscoft Office (Word, PowerPoint, Excel) Firefox, Safari, iTunes
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