Department of Chemistry & Biochemistry
University of Delaware
Newark DE, 19716
B.S., National Taiwan University 1983,
Ph.D., New York Unviersity 1990
The mechanism of homologous recombination:
Our long-term research objective is to study the mechanisms of homologous recombination in eukaryotic systems. Genetic recombination is the molecular process by which new combinations of the genetic material are generated. While homologous recombination is one of the most important biochemical processes in cell, its molecular mechanism, especially in eukaryotes, is still largely unknown. We are interested in homologous recombination not only because of the intellectual challenges it presents, but also on the insights it may bring to the mechanism of the repair of double strand breaks in DNA. Basic understanding of recombination process may lead to the development of new antibacterial and anticancer agents. Currently, we are focusing on two related areas: (1) the mechanism of DNA homology search and strand transfer promoted by the human recombinational proteins, Rad51 and Rad54. (2) The purification and characterization of human Rad51 paralogs, Rad51 B, C, D, and XRCC 2, 3. Our ultimate goal is to understand the function and mechanism of each of these human proteins in homologous recombination.
Large scale genomic monitoring and profiling using a dna-based matrix:
We are developing a new method consisting of separating large library of oligonucleotide DNA in a 2-D matrix that is capable of monitoring or profiling the existence of any input DNA or RNA samples. This 2-D matrix would be sensitive enough that provides unprecedented capacity, and sensitivity. For example, human genomic DNA can be used as the input, and the hybridization signatures of these DNA to these matrixes are the output that represents the genomic “signature” of this individual. Due to the high sensitivity of the matrix, this would detect minor differences of two closely related input DNAs. Therefore, generate genomic and gene expression signatures of specific organism, cell type, or tissue that are indicative of different species, phenotypes, or diseases.
The structure & function of kinetoplast dna networks:
Kinetoplast DNA (kDNA) is the mitochondrial DNA of trypanosomatids and related protozoan parasites such as those of the genera Trypanosoma, Leishmania, and Crithidia. The structure of kDNA is unique in nature, consisting of a network of thousands of topologically interlocked circles. The goals of this project are to understand and define the overall topological structure of the unique catenated kinetoplast DNA network to explain its basic biological properties. In addition, we wish to study the enzymatic activities of mitochondrial type II topoisomerases that are essential for the stability and the replication of this unique network structure.